A relationship of n-acetylaspartate biosynthesis to neuronal protein synthesis / D. D. Clarke, S. Greenfield, E. Dicker, L.J. Tirri, and E.J. Ronan Chemistry Department, Fordham University, Bronx, NY 10458, U.S.A.
A detailed time study of the incorporation of label from sodium-[1-14C]acetate, [1-14C]ethanol, and [2-14C]glucose into the aspartyl moiety of N-acetylaspartic acid (NAA) was conducted. As expected the specific activity of aspartate increased rapidly with time and peaked within 15-20 min after which it fell sharply: but significantly that of the aspartyl moiety of NAA rose very slowly even after the specific activity of aspartate had fallen to less than 1 per cent of the peak values. A rat brain microsomal free supernatant preparation was shown enzymatically to incorporate label from sodium-[l-14C]acetate into the t-RNA fraction from which was isolated N-[1-14C]acetylaspartic acid. From these observations we were inclined to speculate that NAA-t-RNA may serve as an initiator of neuronal protein synthesis
Clarke, Donald Dudley PhD; Greenfield, S.; Dicker, E.; and Tirri, L. J., "A relationship of n-acetylaspartate biosynthesis to neuronal protein synthesis / D. D. Clarke, S. Greenfield, E. Dicker, L.J. Tirri, and E.J. Ronan Chemistry Department, Fordham University, Bronx, NY 10458, U.S.A." (1975). Chemistry Faculty Publications. 69.